Application
IHC-P analysis of human lymph node lymphoma using CD15 antibody [C3D-1].| Product Name | CD15 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Monoclonal Mouse Antibody to Human Granulocyte Associated Antigen CD15The specificity of this antibody is similar to antibodies Leu-M1, TU 9 and VIM-D5, recognizing an epitope involving the carbohydrate sequence 3-fucosyl-N-acetyllacetosamine. This antibody reacts with an antigen located on mature granulocytes and on Hodgkin and Reed-Sternberg cells. |
| Immunogen | Purified neutrophils from normal human peripheral blood. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Clone Number | C3D-1 |
| Host Species | Mouse |
| Tested Applications | IHC-P |
| This antibody may be diluted to a titer of 1:10-1:25 in an ABC method, the final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 1mM EDTA buffer, pH 8.0 or Tris-EDTA buffer, pH 9.0 prior to immunostaining.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CD15 ELFT FCT3A FUC-TIV FUTIV LeX SSEA-1 SSEA 1 FUC TIV SSEA1 FUCTIV |
|---|---|
| Target | CD15 |
| Cellular Localization | Cell membrane |
| Light Chain Type | Kappa |
Application
IHC-P analysis of human lymph node lymphoma using CD15 antibody [C3D-1].| Positive Control | Tonsil |
|---|---|
| Application Notes | This antibody may be diluted to a titer of 1:10-1:25 in an ABC method, the final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 1mM EDTA buffer, pH 8.0 or Tris-EDTA buffer, pH 9.0 prior to immunostaining.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
