Application
FACS analysis of human peripheral blood lymphocytes using CD16 antibody [DJ130c].| Product Name | CD16 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [DJ130c] to CD16This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other other antibody-dependent responses. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | DJ130c |
| Host Species | Mouse |
| Tested Applications | FACSIHC-FIHC-P |
| Flow Cytometry: 1/20 - 1/200 Optimal dilution / concentration should be determined by the end user.: |
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| Species Reactivity | HumanMacaque Monkey |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FCRIIIA FCRIII FCR-10 FCGRIII FCG3 CD16A FCGR3A FCGR3 IGFR3 Fc fragment of IgG low affinity IIIa receptor (CD16a) |
|---|---|
| Target | CD16 |
| Tissue Specificity | This antibody recognizes both CD16a and CD16b and has been demonstrated to cross-react with CD16 from rhesus macaques, Macaca mulatta (Xu et al. 2012) |
Application
FACS analysis of human peripheral blood lymphocytes using CD16 antibody [DJ130c].| Application Notes | Flow Cytometry: 1/20 - 1/200 Optimal dilution / concentration should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Storage Buffer | Phosphate buffered saline containing 0.09% Sodium Azide (NaN3) |
