Application
Surface staining of human peripheral blood cells with anti-human CD3 (MEM-57) FITC . Cells in the lymphocyte gate were used for analysis.| Product Name | CD3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [MEM-57] to CD3T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. |
| Immunogen | Human thymocytes and T lymphocytes |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | MEM-57 |
| Host Species | Mouse |
| Tested Applications | FACSIP |
| For FACS: Use at a concentration of 2-5ug/ml. Positive control: Peripheral Blood Lymphocytes, Jurkat human leukemia T cell line. For FuncS: Use at an assay dependant dilution. Activates T cells in vitro. GTX28090 has a strong mitogenic effect on peripheral T lymphocytes. IP: Use at an assay dependant concentration. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | T3E 186740 P09693 T3D 916 CD3-GAMMA 917 CD3GAMMA TCRE P20963 186830 186780 P04234 CD3ZETA CD3H CD3Q CD3E CD3 CD3-DELTA TCRZ 919 CD3Z CD3G 915 CD3-ZETA 186790 T3G CD3D P07766 CD3DELTA CD3 ZETA CD3 GAMMA CD3 DELTA |
|---|---|
| Target | CD3 |
| Tissue Specificity | This antibody reacts with epsilon-gamma and epsilon-delta dimers of human CD3 complex, a part of a bigger multisubunit complex of the T cell receptor for antigen (CD3/TCR) expressed on peripheral blood T lymphocytes and mature thymocytes. It reacts more strongly with blood gamma-delta T lymphocytes than with alpha-beta T lymphocytes. |
| Cellular Localization | Type I membrane protein. |
Application
Surface staining of human peripheral blood cells with anti-human CD3 (MEM-57) FITC . Cells in the lymphocyte gate were used for analysis.| Application Notes | For FACS: Use at a concentration of 2-5ug/ml. Positive control: Peripheral Blood Lymphocytes, Jurkat human leukemia T cell line. For FuncS: Use at an assay dependant dilution. Activates T cells in vitro. GTX28090 has a strong mitogenic effect on peripheral T lymphocytes. IP: Use at an assay dependant concentration. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
