Application
Frozen section of human tonsil stained with Monoclonal AntiCD3, clone no. UCHT-1 using the APAAP method.| Product Name | CD3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [UCHT1] to CD3T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. |
| Immunogen | Human infant thymocytes and Sezary cells |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | UCHT1 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHCIHC-FIPRIA |
| This antibody is not suitable for use on paraffin sections.: IHC-F:1:50~1:100 ICC/IF:1:100~1:500 IP:1:100~1:500 |
|
| Species Reactivity | HumanMonkeyPigChimpanzee |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | T3E 186740 P09693 T3D 916 CD3-GAMMA 917 CD3GAMMA TCRE P20963 186830 186780 P04234 CD3ZETA CD3H CD3Q CD3E CD3 CD3-DELTA TCRZ 919 CD3Z CD3G 915 CD3-ZETA 186790 T3G CD3D P07766 CD3DELTA CD3 ZETA CD3 GAMMA CD3 DELTA |
|---|---|
| Target | CD3 |
| Tissue Specificity | Detects the non-glycosylated epsilon chain of CD3 (MW 20kD) which is closely associated on the lymphocyte cell surface with the T cell antigen receptor (TCR).The antibody reacts with T cells in the thymus, bone marrow, and peripheral blood and is able to induce proliferation of mature thymocytes and T cells with, and without the presence of IL-2.The majority of T cell neoplasms are positive with this antibody. |
| Cellular Localization | Type I membrane protein. |
| Light Chain Type | kappa |
Application
Frozen section of human tonsil stained with Monoclonal AntiCD3, clone no. UCHT-1 using the APAAP method.| Application Notes | This antibody is not suitable for use on paraffin sections.: IHC-F:1:50~1:100 ICC/IF:1:100~1:500 IP:1:100~1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
