Application
IHC-P analysis of human tonsil stained using CD3 epsilonantibody.| Product Name | CD3 epsilon antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Polyclonal Antibody to CD3 e ( Early T Cell Marker )This antibody reacts with a 20 kD protein. This antibody reacts with intracytoplasmic portion of the CD3 antigen expressed by T cells. It stains human T cells in both the cortex and medulla of the thymus and in peripheral lymphoid tissues. This antibody cross reacts with baboon, monkey, horse, dog and cat. |
| Immunogen | A synthetic 13-mer peptide corresponding to aa 156-168 of the epsilon chain of human CD3 protein. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-P |
| This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CD3e T3E 916 TCRE 186830 P07766 |
|---|---|
| Target | CD3e |
| Cellular Localization | Cell membrane |
Application
IHC-P analysis of human tonsil stained using CD3 epsilonantibody.| Positive Control | Tonsil |
|---|---|
| Application Notes | This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
