Application
Detection of CD34 in human chronic myeloid leukemia cell line MOLM-7 with anti-human CD34 [4H11[APG]]: PE .Application
Surface staining of Kg-1a human acute myelogenous leukemia cell line with anti-human CD34 [4H11[APG]]: FITC . Total viable cells were used for analysis.| Product Name | CD34 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [4H11(APG)] to CD34CD34 is possibly a adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction. |
| Immunogen | Tissue / cell preparation (Human). (Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 4H11(APG) |
| Host Species | Mouse |
| Tested Applications | BlockingFACSICC/IF |
| BL: Use at an assay dependant dilution. Completely blocks the binding of Class II monoclonal antibody QBEnd10 and Class III monoclonal antibodies BIRMA K3 and 8G12 in cross-blocking studies on a KG1a cell line. FACS: Use at an assay dependant concentration. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CD34 P28906 947 142230 |
|---|---|
| Target | CD34 |
| Tissue Specificity | This antibody reacts with Class III epitope on CD34 antigen. |
| Cellular Localization | Type I membrane protein. |
Application
Detection of CD34 in human chronic myeloid leukemia cell line MOLM-7 with anti-human CD34 [4H11[APG]]: PE .
Application
Surface staining of Kg-1a human acute myelogenous leukemia cell line with anti-human CD34 [4H11[APG]]: FITC . Total viable cells were used for analysis.| Application Notes | BL: Use at an assay dependant dilution. Completely blocks the binding of Class II monoclonal antibody QBEnd10 and Class III monoclonal antibodies BIRMA K3 and 8G12 in cross-blocking studies on a KG1a cell line. FACS: Use at an assay dependant concentration. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
