Application
Immunofluorescent analysis of CFTR in HeLa cells. CFTR staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with CFTR antibody [CF3] at a dilution of 1:100-1:200 over night at 4 °C, washed with PBS and incubated with a proper secondary antibody. Images were taken at 60X magnification.Application
Immunohistochemistry was performed on cancer biopsies of deparaffinized human colon carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with or without CFTR antibody [CF3] overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.| Product Name | CFTR antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [CF3] to CFTRThis gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily that is involved in multi-drug resistance. The encoded protein functions as a chloride channel and controls the regulation of other transport pathways. Mutations in this gene are associated with the autosomal recessive disorders cystic fibrosis and congenital bilateral aplasia of the vas deferens. Alternatively spliced transcript variants have been described, many of which result from mutations in this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | Synthetic Peptide: G(103) R I I A S Y D P D N K E E R(117) |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Clone Number | CF3 |
| Host Species | Mouse |
| Tested Applications | BlockingFACSICC/IFIHCIHC-PIPWB |
| ICC/IF: 1:50 - 1:500: |
|
| Species Reactivity | HumanMouseChickenRat |
| Concentration | 1mg/ml |
| Gene Synonyms | CFTR/MRP cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C member 7) dJ760C5.1 ABCC7 CF MRP7 ABC35 TNR-CFTR |
|---|---|
| Target | CFTR |
Application
Immunofluorescent analysis of CFTR in HeLa cells. CFTR staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with CFTR antibody [CF3] at a dilution of 1:100-1:200 over night at 4 °C, washed with PBS and incubated with a proper secondary antibody. Images were taken at 60X magnification.
Application
Immunohistochemistry was performed on cancer biopsies of deparaffinized human colon carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with or without CFTR antibody [CF3] overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.| Application Notes | ICC/IF: 1:50 - 1:500: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20° C, Avoid Freeze/Thaw Cycles |
| Storage Buffer | Ascites containing 0.05 % sodium azide |
