| Product Name | Clathrin Light chain antibody [3F133] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [3F133] to Clathrin light chainVarious cell types express both forms of clathrin LC in different relative levels. LCs are randomly distributed on clathrin triskelion, resulting in four types of clathrin triskelion with different LC composition (3LCa, 2LCa 1LCb, 2LCb-1LCa, 3LCb). In most cells, the LCa form is dominant. In contrast, cells with a regulated secretory pathway contain more LCb than LCa, suggesting that LCb may play a role in the specialized clathrin functions in these cells. Neuronal cells, express splicing variants of both LCa and LCb. A distinguishing feature of the LCb light chain is its ability to be phosphorylated by a casein kinase, at a site which is not present in the LCa sequence. |
| Immunogen | Synthetic peptide: EEDPAAAFLAQQESEIAGIEND, corresponding to amino acids 23-44 of the conserved regions of Cow Clathrin Light Chain. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 3F133 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PIPWB |
| IF: Use at an assay dependent dilution. IHC-P: Use at a concentration of 1 - 2 μg/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. IP: Use at an assay dependent dilution with protein A. WB: Use at a concentration of 1 - 2 μg/ml for 2hrs at RT. Predicted molecular weight: 28 kDa. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMouseRatZebra FishFishXenopusBovine |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | 118970, P09497, CLTA, P09496, LCB, 118960, CLTB, LCA, Clathrin Light chain, 1211, 1212, CLTA2, MGC140767 |
|---|---|
| Target | Clathrin Light chain |
| Tissue Specificity | reacts with both LCa and LCb. |
| Cellular Localization | Cell Membrane and Cytoplasmic |
| Positive Control | Placenta |
|---|---|
| Application Notes | IF: Use at an assay dependent dilution. IHC-P: Use at a concentration of 1 - 2 μg/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. IP: Use at an assay dependent dilution with protein A. WB: Use at a concentration of 1 - 2 μg/ml for 2hrs at RT. Predicted molecular weight: 28 kDa. Optimal dilutions/concentrations should be determined by the end user. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.09% Sodium Azide; Constituents: 0.2% BSA, 10mM PBS. pH 7.4 |
