| Product Name | Clathrin Light chain antibody [3F133] (Functional Grade) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [3F133] to Clathrin light chain - BSA and Azide freeVarious cell types express both forms of clathrin LC in different relative levels. LCs are randomly distributed on clathrin triskelion, resulting in four types of clathrin triskelion with different LC composition (3LCa, 2LCa 1LCb, 2LCb-1LCa, 3LCb). In most cells, the LCa form is dominant. In contrast, cells with a regulated secretory pathway contain more LCb than LCa, suggesting that LCb may play a role in the specialized clathrin functions in these cells. Neuronal cells, express splicing variants of both LCa and LCb. A distinguishing feature of the LCb light chain is its ability to be phosphorylated by a casein kinase, at a site which is not present in the LCa sequence. |
| Immunogen | Synthetic peptide: EEDPAAAFLAQQESEIAGIEND, corresponding to amino acids 23-44 of the conserved regions of Cow Clathrin Light Chain. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 3F133 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PIPWB |
| ICC: Use at an assay dependent dilution. | |
| Species Reactivity | HumanMouseRatZebra FishFishXenopusBovine |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | 118970, P09497, CLTA, P09496, LCB, 118960, CLTB, LCA, Clathrin Light chain, 1211, 1212, CLTA2, MGC140767 |
|---|---|
| Target | Clathrin Light chain |
| Tissue Specificity | Reacts with both LCa and LCb. |
| Cellular Localization | Cell Membrane and Cytoplasmic |
| Positive Control | Placenta |
|---|---|
| Application Notes | ICC: Use at an assay dependent dilution. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4ºC for short-term only. For long-term storage store and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20ºC, stable for at least 12 months at -20ºC. |
| Storage Buffer | 10mM PBS (pH 7.4), with no preservatives |
