| Product Name | Clathrin Light chain antibody [CON.1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [CON.1] to Clathrin light chainVarious cell types express both forms of clathrin LC in different relative levels. LCs are randomly distributed on clathrin triskelion, resulting in four types of clathrin triskelion with different LC composition (3LCa, 2LCa 1LCb, 2LCb-1LCa, 3LCb). In most cells, the LCa form is dominant. In contrast, cells with a regulated secretory pathway contain more LCb than LCa, suggesting that LCb may play a role in the specialized clathrin functions in these cells. Neuronal cells, express splicing variants of both LCa and LCb. A distinguishing feature of the LCb light chain is its ability to be phosphorylated by a casein kinase, at a site which is not present in the LCa sequence. |
| Immunogen | Synthetic peptide, corresponding to amino acids 23-44 of Cow Clathrin Light Chain. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | CON.1 |
| Host Species | Mouse |
| Tested Applications | PurifyICC/IFIHCWB |
| AP: Use at a concentration of 1 - 2 μg/ml. ICC: Use at a concentration of 1 - 2 μg/ml. IHC: Use at a concentration of 1 - 2 μg/ml. WB: Use at a concentration of 1 - 2 μg/ml. Detects a doublet at approximately 25-36 kDa, a doublet at approximately 56 kDa and possibly additional bands (lower and higher molecular weight). Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMouseRatBovine |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | 118970, P09497, CLTA, P09496, LCB, 118960, CLTB, LCA, Clathrin Light chain, 1211, 1212, CLTA2, MGC140767 |
|---|---|
| Target | Clathrin Light chain |
| Tissue Specificity | Monoclonal Anti-Clathrin Light Chain reacts specifically with the clathrin light chain. The epitope recognized by the antibody resides within amino acids 23-44 of bovine clathrin light chains, LCa and LCb. This region is not involved in heavy chain binding, and is completely conserved in all mammals. |
| Cellular Localization | Cell Membrane and Cytoplasmic |
| Application Notes | AP: Use at a concentration of 1 - 2 μg/ml. ICC: Use at a concentration of 1 - 2 μg/ml. IHC: Use at a concentration of 1 - 2 μg/ml. WB: Use at a concentration of 1 - 2 μg/ml. Detects a doublet at approximately 25-36 kDa, a doublet at approximately 56 kDa and possibly additional bands (lower and higher molecular weight). Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 0.01M PBS. pH 7.4 |
