Application
anti collagen II antibody (1:400, 45 min RT) showed moderate staining of in FFPE human bronchiolar cartilage (shown in image). Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval.| Product Name | Collagen II antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [5B2.5] to Collagen IICollagen II is highly specific to type II collagen and shows no cross-reaction with types I, III, IV, V, VI, IX, X, or XI. In Western blotting, 5B2.5 MAb reacts with the TCA fragment of lathyritic type II collagen after digestion with mammalian collagenase. |
| Immunogen | A purified preparation of lathyritic type II collagen from embryonic chicken sternum. |
| Conjugation | Agarose |
| Modification | Acetylated |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 5B2.5 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| For ICC/IF: Use at an assay dependent concentration. For WB: Use at a dilution of 1-2μg/ml for 2hrs at RT. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 395069 Collagen II COL2A1 |
|---|---|
| Target | Collagen II |
| Tissue Specificity | This antibody is highly specific to type II collagen and shows no cross-reaction with types I, III, IV, V, VI, IX, X, or XI. In Western blotting, this antibody reacts with the TCA fragment of lathyritic type II collagen after digestion with mammalian collagenase. It does not react with pepsin-digested type II collagen. |
| Cellular Localization | Cartilage matrix. |
| Light Chain Type | kappa |
Application
anti collagen II antibody (1:400, 45 min RT) showed moderate staining of in FFPE human bronchiolar cartilage (shown in image). Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval.| Positive Control | Cartilage. |
|---|---|
| Application Notes | For ICC/IF: Use at an assay dependent concentration. For WB: Use at a dilution of 1-2μg/ml for 2hrs at RT. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide |
