Application
Western blot analysis of CX3CL1 in 0.25 μg of recombinant human CX3CL1 protein. Protein was transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membrane was probed with CX3CL1 antibody [4C5] at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1% Tween-20 and probed with a HRP-conjugated secondary antibody. Membrane was washed and chemiluminescent detection was performed.Application
ELISA analysis of human CX3CL1 was performed by coating a blank 96-well microtiter plate with 1μg/ml of recombinant CX3CL1 protein and incubating overnight. The plate was aspirated and blocked with block solution. The plate was washed then incubated for 2 hours with serial dilutions (10μg/ml) of CX3CL1 antibody [4C5]. The plate was washed and incubated with a proper secondary antibody, washed again, and 100μl per well of TMB substrate was added for 30 minutes at room temperature in the dark. The reaction was stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550nm.| Product Name | CX3CL1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [4C5] to CX3CL1 |
| Immunogen | Non-glycosylated, polypeptide chain containing 76 amino acids of chemokine domain expressed in E. coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | 4C5 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| WB: 1:1000 Optimal dilution / concentration should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Gene Synonyms | neurotactin NTN C3Xkine SCYD1 CXC3C CXC3 chemokine (C-X3-C motif) ligand 1 CX3CL1 fractalkine ABCD-3 NTT |
|---|---|
| Target | Fractalkine |
Application
Western blot analysis of CX3CL1 in 0.25 μg of recombinant human CX3CL1 protein. Protein was transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membrane was probed with CX3CL1 antibody [4C5] at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1% Tween-20 and probed with a HRP-conjugated secondary antibody. Membrane was washed and chemiluminescent detection was performed.
Application
ELISA analysis of human CX3CL1 was performed by coating a blank 96-well microtiter plate with 1μg/ml of recombinant CX3CL1 protein and incubating overnight. The plate was aspirated and blocked with block solution. The plate was washed then incubated for 2 hours with serial dilutions (10μg/ml) of CX3CL1 antibody [4C5]. The plate was washed and incubated with a proper secondary antibody, washed again, and 100μl per well of TMB substrate was added for 30 minutes at room temperature in the dark. The reaction was stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550nm.| Application Notes | WB: 1:1000 Optimal dilution / concentration should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20° C, Avoid Freeze/Thaw Cycles |
| Storage Buffer | PBS containing 50 % glycerol and 0.05 % sodium azide |
