Application
Formalin fixed paraffin embedded human breast carcinoma stained with Cytokeratin 18 antibody (cat. # ).Application
Intracellular flow cytometry analysis of cytokeratin expression in HeLa human cervix carcinoma cell line using anti-Cytokeratin 18 (C-04): FITC. Overlay with Isotype mouse IgG1 control.Application
Immunohistochemistry staining of human kidney (paraffin-embedded sections) with anti-Cytokeratin 18 (C-04).Application
Immunohistochemistry staining of human colon (paraffin-embedded sections) with anti-Cytokeratin 18 (C-04).| Product Name | Cytokeratin 18 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [DC 10] to Cytokeratin 18Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.Cytokeratin 18 belongs to type I family (acidic cytokeratins). |
| Immunogen | Human breast carcinoma cell line PMC-42 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | DC10 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHC-PIPWB |
| Positive control:: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CYK18 K18 |
|---|---|
| Target | Cytokeratin 18 |
| Tissue Specificity | The antibody DC-10 reacts with Cytokeratin 18 (45 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues. |
Application
Formalin fixed paraffin embedded human breast carcinoma stained with Cytokeratin 18 antibody (cat. # ).
Application
Intracellular flow cytometry analysis of cytokeratin expression in HeLa human cervix carcinoma cell line using anti-Cytokeratin 18 (C-04): FITC. Overlay with Isotype mouse IgG1 control.
Application
Immunohistochemistry staining of human kidney (paraffin-embedded sections) with anti-Cytokeratin 18 (C-04).
Application
Immunohistochemistry staining of human colon (paraffin-embedded sections) with anti-Cytokeratin 18 (C-04).| Positive Control | WB: HeLa, MCF-7; IHC-P: colon, skin; ICC/IF and ELISA: MCF-7; |
|---|---|
| Application Notes | Positive control:: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15 mM sodium azide |
