Application
Dicer antibody detects Dicer protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Non-transfected (–) and transfected (+) SW480 whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Dicer antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DicerThis gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Dicer. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Pig, Chicken, Rhesus Monkey, BovineZebrafish (93%), Xenopus laevis (92%), Pig (99%), Chicken (96%), Rhesus Monkey (100%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DICER1 HERNA DCR1 dicer 1 ribonuclease type III MNG1 |
|---|---|
| Target | Dicer |
| Molecular Weight(MW) | 219 kDa (note) (5) |
| Cellular Localization | Cytoplasm |
Application
Dicer antibody detects Dicer protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Non-transfected (–) and transfected (+) SW480 whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , 293T , A431 , HeLa , HepG2 , MCF-7 , SW480 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
