Application
Detection of human DNA polymerase delta by using anti-pol delta monoclonal antibody [607] in HeLa whole cell extract. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: BCL-1 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Product Name | DNA polymerase delta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [607] to DNA polymerase deltaDNA polymerase d (delta) functions in DNA replication and DNA repair. It also possesses 3' to 5' exonuclease activity. In leading strand synthesis it requires the processivity factor, PCNA. It is also possibly involved in the completion of DNA synthesis during Okazaki fragment elongation following the initiation of synthesis by the DNA polymerase a/primase complex |
| Immunogen | Recombinant protein encoding amino acids 1-140 of the large subunit of human DNA polymerase d. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | 607 |
| Host Species | Mouse |
| Tested Applications | WB |
| For WB: Use at a concentration of 1:1,000. Predicted molecular weight: 130 kDa. Optimal dilutions/concentrations should be determined by the reseracher.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | P28340 DNA polymerase delta 174761 5424 POLD1 POLD |
|---|---|
| Target | DNA polymerase delta |
Application
Detection of human DNA polymerase delta by using anti-pol delta monoclonal antibody [607] in HeLa whole cell extract. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: BCL-1 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Positive Control | HeLa , BCL-1 |
|---|---|
| Application Notes | For WB: Use at a concentration of 1:1,000. Predicted molecular weight: 130 kDa. Optimal dilutions/concentrations should be determined by the reseracher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containing no preservatives |
