Mouse Monoclonal to Human DNA Polymerase Delta [6169]DNA polymerase d (delta) functions in DNA replication and DNA repair. It also possesses 3' to 5' exonuclease activity. In leading strand synthesis it requires the processivity factor, PCNA. It is also possibly involved in the completion of DNA synthesis during Okazaki fragment elongation following the initiation of synthesis by the DNA polymerase a/primase complex
Immunogen
Recombinant protein encoding amino acids 1-140 of the large subunit of human DNA polymerase d.
Key Feature
Clonality
Monoclonal
Isotype
IgG
Clone Number
6196
Host Species
Mouse
Tested Applications
WB
For WB: Use at a dilution of 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher.:
Species Reactivity
Human
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
174761 5424 POLD P28340 DNA polymerase delta POLD1
Target
DNA polymerase delta
Application
Application
Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with DNA polymerase delta antibody [6196] diluted at 1:1000.
Positive Control
HeLa
Application Notes
For WB: Use at a dilution of 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
Storage Buffer
Phosphate-buffered saline, pH 7.2, containing no preservatives