Application
DNMT3B antibody detects DNMT3B protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNMT3B protein stained by DNMT3B antibody diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 μm.Application
DNMT3B antibody detects DNMT3B protein at nucleus on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. DNMT3B antibody diluted at 1:250.Application
Immunoprecipitation of DNMT3B protein from K562 nuclear extracts using 5 μg of DNMT3B antibody . Western blot analysis was performed using DNMT3B antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
DNMT3B antibody detects DNMT3B protein by western blot analysis. HeLa whole cell extracts and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT3B antibody diluted by 1:500.| Product Name | DNMT3B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DNMT3B (DNA (cytosine-5-)-methyltransferase 3 beta)CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Six alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human DNMT3B. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | M.HsaIIIB ICF DNA (cytosine-5-)-methyltransferase 3 beta ICF1 |
|---|---|
| Target | DNMT3B |
| Molecular Weight(MW) | 96 kDa (note) (4) |
| Cellular Localization | Nucleus |
Application
DNMT3B antibody detects DNMT3B protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNMT3B protein stained by DNMT3B antibody diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 μm.
Application
DNMT3B antibody detects DNMT3B protein at nucleus on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. DNMT3B antibody diluted at 1:250.
Application
Immunoprecipitation of DNMT3B protein from K562 nuclear extracts using 5 μg of DNMT3B antibody . Western blot analysis was performed using DNMT3B antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
DNMT3B antibody detects DNMT3B protein by western blot analysis. HeLa whole cell extracts and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT3B antibody diluted by 1:500.| Positive Control | HeLa , HeLa nuclear |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
