Application
ChIP ImageChIP was performed with GDP extract and either control IgG (5 μg/IP) or DNMT3B antibody at a titration of 1, 2, and 5 μg per ChIP experiment. The precipitated DNA was detected by qPCR with two different primer pairs for the PRRX1 gene, used as a positive control target.| Product Name | DNMT3B antibody - ChIP grade |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to DNMT3B |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAChIP |
| ChIP (1 µg/ChIP), ELISA (1:1000): |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ICF M.HsaIIIB DNA (cytosine-5-)-methyltransferase 3 beta ICF1 |
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Application
ChIP ImageChIP was performed with GDP extract and either control IgG (5 μg/IP) or DNMT3B antibody at a titration of 1, 2, and 5 μg per ChIP experiment. The precipitated DNA was detected by qPCR with two different primer pairs for the PRRX1 gene, used as a positive control target.| Application Notes | ChIP (1 µg/ChIP), ELISA (1:1000): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.05% azide and 0.05% ProClin 300. |
