Application
The image above is a xenograph of human cancer cells inplanted into a nude mouse and allowed to grow for about three weeks. Notice only the humancancer cells expressed this antigen.Application
Western blot analysis of DR5 in HeLa (H) and K562 (K) whole cell lysates with Anti-DR5 (Cat. No. ) at 1:500 dilution.| Product Name | DR5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to DR5Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including tumor necrosis factor (TNF) and Fas ligand in the TNF family through their death domain containing receptors, TNF receptor 1 (TNFR1) and Fas, respectively. Another member in the TNF family has been identified and designated TRAIL (for TNF related apoptosis inducing ligand) and Apo2L (for Apo2 ligand). Receptors for TRAIL include two death domain containing receptors, DR4 and DR5, as well as two decoy receptors, DcR1 and DcR2, lacking the intracellular signaling death domain. DR5 contains an intracellular death domain. This receptor can be activated by tumor necrosis factor related apoptosis inducing ligand (TNFSF10/TRAIL/APO2L), and transduces apoptosis signal. Studies with FADD deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. |
| Immunogen | Synthetic peptide, corresponding to amino acids 388-407 of Human DR5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIHC-PWB |
| Recommended dilutions: WB: 1/250 - 1/500. Detects a band of approximately 60 kDa (predicted molecular weight: 47.8 kDa). Can be blocked with DR5 (C-term) peptide (human) (GTX28449). IHC-P: 1/100. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | HumanMouse |
| Concentration | 0.5mg/ml |
| Gene Synonyms | TNFRSF10B KILLER/DR5 8795 TRICK2 O14763 TRAILR2 KILLER TRICK2A TRICKB 275355 TRAIL-R2 ZTNFR9 DR5 TRICK2B TRAIL R2 |
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Application
The image above is a xenograph of human cancer cells inplanted into a nude mouse and allowed to grow for about three weeks. Notice only the humancancer cells expressed this antigen.
Application
Western blot analysis of DR5 in HeLa (H) and K562 (K) whole cell lysates with Anti-DR5 (Cat. No. ) at 1:500 dilution.| Application Notes | Recommended dilutions: WB: 1/250 - 1/500. Detects a band of approximately 60 kDa (predicted molecular weight: 47.8 kDa). Can be blocked with DR5 (C-term) peptide (human) (GTX28449). IHC-P: 1/100. Optimal dilutions/concentrations should be determined by the end user.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide |
