| Product Name | E-Cadherin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [67A4] to E CadherinOne of the epithelial cell adhesion molecules, E Cadherin, plays an important role in the formation of cell-cell contacts in epithelia irrespective their origin form ecto-, meso- or endodermal tissue. This early adhesion event between epithelial cells is a prerequisite for the assembly of all elements of the junctional complex. Furthermore, E Cadherin plays a crucial role in the maintenance of the epithelial junctional complex and is as such an important molecule in maintaining epithelial integrity. Over 90% of the malignant tumors are carcimomas. One of the prerequisites for the release of carcinoma cells from the primary site might be a defect in intercellular adhesion mediated by the absence of E Cadherin expression. Therefore, the expression of E Cadherin might be an important parameter for the determination of the invasive potential of epithelial neoplasms, and for the transition of a benign to a malignant neoplasm. |
| Immunogen | The immunogen is “Human thoracic duct lymphocytes” expressed full length E-Cadherin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 67A4 |
| Host Species | Mouse |
| Tested Applications | IHC-F |
| IHC: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Suitable for frozen tissue sections only: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Arc-1 CDHE 137215 CDH1 P12830 UVO 999 ECAD E Cadherin LCAM Arc1 Arc 1 |
|---|---|
| Target | E Cadherin |
| Tissue Specificity | This antibody is specific for the 123 kDa E-cadherin molecule, expressed on non-neuronal epithelial cells. This antibody reacts with tumor cell lines such as breast carcinoma cells. |
| Application Notes | IHC: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Suitable for frozen tissue sections only: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | Tissue culture supernatant with sodium azide |
