Application
Immunohistochemical analysis of paraffin-embedded gastric cancer tissues (left) and lung cancer tissues (right) using CDH1 mouse mAb with DAB staining.Application
Western blot analysis using CDH1 mouse mAb against LNCAP (1)，A431 (2), DU145 (3), PC-3 (4), MCF-7 (5), PC-12 (6), NIH/3T3 (7), C6 (8) and COS7 (9) cell lysate.Application
Flow cytometric analysis of Hela cells using CDH1 mouse mAb (green) and negative control (purple)| Product Name | E-Cadherin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [7H12] to CDH1This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq, Jul 2008] |
| Immunogen | The immunogen corresponding to human CDH1 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 7H12 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | HumanMouseMonkey |
| Concentration | 1mg/ml |
| Gene Synonyms | CDHE ECAD UVO ARC-1 CD324 CDH1 LCAM |
|---|---|
| Target | CDH1 |
| Molecular Weight(MW) | 135 kDa (note) |
Application
Immunohistochemical analysis of paraffin-embedded gastric cancer tissues (left) and lung cancer tissues (right) using CDH1 mouse mAb with DAB staining.
Application
Western blot analysis using CDH1 mouse mAb against LNCAP (1)，A431 (2), DU145 (3), PC-3 (4), MCF-7 (5), PC-12 (6), NIH/3T3 (7), C6 (8) and COS7 (9) cell lysate.
Application
Flow cytometric analysis of Hela cells using CDH1 mouse mAb (green) and negative control (purple)| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
