Application
Flow cytometric analysis of Hela cells, using anti-HSD17B10 antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HSD17B10 monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-HSD17B10mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-HSD17B10mouse monoclonal antibody. ( Dilution 1:50)Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSD17B10 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSD17B10.Application
Anti-HSD17B10 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with HSD17B10Application
Flow cytometric analysis of Jurkat cells, using anti-HSD17B10 antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).Application
Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-HSD17B10mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded liver tissue using anti-HSD17B10mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-HSD17B10mouse monoclonal antibody. ( Dilution 1:50)| Product Name | ERAB antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [10B4] to ERABFunctions in mitochondrial tRNA maturation. Part of mitochondrial ribonuclease P, an enzyme composed of MRPP1/RG9MTD1, MRPP2/HSD17B10 and MRPP3/KIAA0391, which cleaves tRNA molecules in their 5'-ends. By interacting with intracellular amyloid-beta, it may contribute to the neuronal dysfunction associated with Alzheimer disease (AD). |
| Immunogen | Full length human recombinant protein of human ERAB (NP_004484) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 10B4 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHCIHC-PWB |
| FACS:1:200~1:500 ICC/IF:1:200~1:500 IHC:1:50~1:200 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ABAD 17bHSD10 17b-HSD10 MHBD HSD17B10 ERAB 17b HSD10 |
|---|---|
| Target | ERAB |
| Tissue Specificity | is specific for human ERAB (NP_004484). |
Application
Flow cytometric analysis of Hela cells, using anti-HSD17B10 antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HSD17B10 monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-HSD17B10mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-HSD17B10mouse monoclonal antibody. ( Dilution 1:50)
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSD17B10 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSD17B10.
Application
Anti-HSD17B10 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with HSD17B10
Application
Flow cytometric analysis of Jurkat cells, using anti-HSD17B10 antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
Application
Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-HSD17B10mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded liver tissue using anti-HSD17B10mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-HSD17B10mouse monoclonal antibody. ( Dilution 1:50)| Application Notes | FACS:1:200~1:500 ICC/IF:1:200~1:500 IHC:1:50~1:200 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
