Application
IF usingApplication
Mitochondrial localization of ERAB in human neuroblastoma cells SK-N-SH visualized with MAb 5F3 ) and laser-scanning confocal microscope. A. MAb to mitochondria visualized with Cy-5-conjugated secondary antibodies B. MAb 5F3 to ERAB-FITC-conjugated C. Merged image| Product Name | ERAB antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [5F3] to ERABERAB (Endoplasmic Reticulum beta Amyloid Binding Protein) has been associated with beta Amyloid neurotoxicity. ERAB is an intracellular 27 kDa polypeptide which appears to be upregulated in the brains of Alzheimers Disease patients. |
| Immunogen | Recombinant, full-length Human ERAB protein (fusion protein). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 5F3 |
| Host Species | Mouse |
| Tested Applications | DotELISAICC/IFIHC-FIHC-PWB |
| Dot: Use at a dilution of 1/100 - 1/10000. ELISA: Use at an assay dependent dilution. IF: Use at an assay dependent dilution. IHC-P: Use at a dilution of 1/100 - 1/1000. IHC-Fr: Use at a dilution of 1/100 - 1/1000. WB: Use at a dilution of 1/100 - 1/10000. Predicted molecular weight: 27 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | ABAD 300256 MHBD 3028 17bHSD10 HSD17B10 17b-HSD10 HADH2 ERAB Q99714 17b HSD10 |
|---|---|
| Target | ERAB |
| Tissue Specificity | This antibody detects the recombinant human ERAB protein (27kDa) and recognizes the endogenous ERAB protein in cell extracts with virtually no crossreactivity with other human proteins. |
| Cellular Localization | Mitochondrial |
| Light Chain Type | lambda |
Application
IF using
Application
Mitochondrial localization of ERAB in human neuroblastoma cells SK-N-SH visualized with MAb 5F3 ) and laser-scanning confocal microscope. A. MAb to mitochondria visualized with Cy-5-conjugated secondary antibodies B. MAb 5F3 to ERAB-FITC-conjugated C. Merged image| Application Notes | Dot: Use at a dilution of 1/100 - 1/10000. ELISA: Use at an assay dependent dilution. IF: Use at an assay dependent dilution. IHC-P: Use at a dilution of 1/100 - 1/1000. IHC-Fr: Use at a dilution of 1/100 - 1/1000. WB: Use at a dilution of 1/100 - 1/10000. Predicted molecular weight: 27 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, no preservative added. |
