Application
ERp57 antibody detects ERp57 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ERp57 antibody diluted by 1:10000.Application
ERp57 antibody detects ERp57 protein at endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ERp57 protein stained by ERp57 antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.Application
Sample (30 ug of whole cell lysate) A: Neuro2A B: GL261 C: C8D30 7.5% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using ERp57 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: HepG2 B: HCT116 7.5% SDS PAGE diluted at 1:10000Application
ERp57 antibody detects ERp57 protein at cytoplasm on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. ERp57 antibody diluted at 1:500.Application
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERp57 antibody diluted at 1:500.| Product Name | ERp57 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ERp57 (protein disulfide isomerase family A, member 3)This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ERp57. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Pig, Rabbit, Chicken, Sheep, Rhesus Monkey, Chimpanzee, BovinePig (94%), Rabbit (94%), Chicken (80%), Sheep (91%), Rhesus Monkey (98%), Chimpanzee (100%), Bovine (93%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ER60 antibody ERp57 antibody ERp60 antibody ERp61 antibody GRP57 antibody GRP58 antibody HsT17083 antibody P58 antibody PI-PLC antibody PDIA3 antibody protein disulfide isomerase-associated 3 antibody ER protein 60 antibody 58 kDa glucose-regul |
|---|---|
| Molecular Weight(MW) | 57 kDa (note) (2) |
| Cellular Localization | Endoplasmic reticulum lumen (By similarity) , Melanosome |
Application
ERp57 antibody detects ERp57 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ERp57 antibody diluted by 1:10000.
Application
ERp57 antibody detects ERp57 protein at endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ERp57 protein stained by ERp57 antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.
Application
Sample (30 ug of whole cell lysate) A: Neuro2A B: GL261 C: C8D30 7.5% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using ERp57 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: HepG2 B: HCT116 7.5% SDS PAGE diluted at 1:10000
Application
ERp57 antibody detects ERp57 protein at cytoplasm on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. ERp57 antibody diluted at 1:500.
Application
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERp57 antibody diluted at 1:500.| Positive Control | HepG2 , HCT116 , 293T , A431 , HeLa , Neuro2A , GL261 , C8D30 , PC-12 , Rat2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
