Application
Sample (30 µg of whole cell lysate) A: HepG2 7.5% SDS PAGE ERp57 antibody diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of methanol-fixed HeLa, using ERp57 antibody at 1:200 dilution.Application
Sample (50 µg of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using ERp57 antibody at 1:100 dilution.Application
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ERp57 antibody [N3C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
ERp57 antibody [N3C3] detects ERp57 protein at cytosol on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. ERp57 antibody [N3C3] dilution: 1:500.| Product Name | ERp57 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ERp57 (protein disulfide isomerase family A, member 3)This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ERp57. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Pig, Rabbit, Chicken, Sheep, Rhesus Monkey, Chimpanzee, BovineXenopus laevis (84%), Pig (98%), Rabbit (98%), Chicken (90%), Sheep (95%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (96%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ER60 antibody ERp57 antibody ERp60 antibody ERp61 antibody GRP57 antibody GRP58 antibody HsT17083 antibody P58 antibody PI-PLC antibody PDIA3 antibody protein disulfide isomerase-associated 3 antibody ER protein 60 antibody 58 kDa glucose-regul |
|---|---|
| Molecular Weight(MW) | 57 kDa (note) (2) |
| Cellular Localization | Endoplasmic reticulum lumen (By similarity) , Melanosome |
Application
Sample (30 µg of whole cell lysate) A: HepG2 7.5% SDS PAGE ERp57 antibody diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of methanol-fixed HeLa, using ERp57 antibody at 1:200 dilution.
Application
Sample (50 µg of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using ERp57 antibody at 1:100 dilution.
Application
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ERp57 antibody [N3C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
ERp57 antibody [N3C3] detects ERp57 protein at cytosol on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. ERp57 antibody [N3C3] dilution: 1:500.| Positive Control | Rat2 , HeLa , HepG2 , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
