ChIP was performed with the nuclear extract from E14 mESCs (SUZ +/+ or -/-) and either 2.5 μg of EZH2 antibody or control mouse IgG. The precipitated DNA was detected by QPCR with primer set targeting to HoxA10 or T gene.
ChIP was performed with the nuclear extract from E14 mESCs (SUZ +/+ or -/-) and either 2.5 μg of EZH2 antibody or control mouse IgG. The precipitated DNA was detected by QPCR with primer set targeting to HoxA10 or T gene.
Application Notes
ChIP (2.5 μg/ChIP):
Additional Information
Form
Liquid
Storage Instructions
Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.