Application
Wild-type (WT) and Fascin 1 knownout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Fascin 1 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
FSCN1 antibody detects FSCN1 protein at cytoplasm by immunofluorescent analysis. Sample: HCT116 cells were fixed in 100% MeOH for 5 min. Green: FSCN1 protein stained by FSCN1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
Fascin 1 antibody detects Fascin 1 protein by Western blot analysis. A. 30 µg HeLa whole cell lysate/extract 10 % SDS-PAGE Fascin 1 antibody dilution: 1:1000Application
Fascin 1 antibody detects Fascin 1 protein at cytosol on mouse ovary by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. Fascin 1 antibody dilution: 1:500.| Product Name | Fascin 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Fascin 1 (fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus))Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Probably involved in the assembly of actin filament bundles present in microspikes, membrane ruffles, and stress fibers. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Fascin 1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, BovineRat (97%), Pig (94%), Bovine (94%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | SNL FAN1 HSN fascin homolog 1 actin-bundling protein (Strongylocentrotus purpuratus) FSCN1 p55 |
|---|---|
| Target | Fascin 1 |
Application
Wild-type (WT) and Fascin 1 knownout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Fascin 1 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
FSCN1 antibody detects FSCN1 protein at cytoplasm by immunofluorescent analysis. Sample: HCT116 cells were fixed in 100% MeOH for 5 min. Green: FSCN1 protein stained by FSCN1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Fascin 1 antibody detects Fascin 1 protein by Western blot analysis. A. 30 µg HeLa whole cell lysate/extract 10 % SDS-PAGE Fascin 1 antibody dilution: 1:1000
Application
Fascin 1 antibody detects Fascin 1 protein at cytosol on mouse ovary by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. Fascin 1 antibody dilution: 1:500.| Positive Control | HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
