Application
IHC-P analysis of human heart tissue using Gli2 antibody at 4.0-8.0μg/ml.Application
IHC-P analysis of human intestine tissue using Gli2 antibody at 4.0-8.0μg/ml.Application
WB analysis of HepG2 cells using Gli2 antibody at 1.0μg/ml.Application
IHC-Fr analysis of mouse gut tissue using Gli2 antibody at 1:50. Green : GL2 Red : Neural crest cell. Fixed tissue was treated with 0.1% Triton X-100 for 4-6 hours at room temperature or overnight at 4°C, washed in PBS, incubated with primary antibodies for 4-6 hours at room temperature or overnight at 4°C, washed in PBS, and then stained with secondary antibody (Goat Anti-Rabbit Cy3) for 4-6 hours at room temperature or overnight at 4°C.Application
WB analysis of Jurkat cells using Gli2 antibody at 2.5μg/ml. Lane A : primary antibody Lane B : primary antibody + blocking peptide| Product Name | Gli2 antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Gli2This gene encodes a protein which belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B. [provided by RefSeq, Jul 2008] |
| Immunogen | A synthetic peptide corresponding to an Internal region of Human Gli2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-FIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Human: 100% |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | GLI2 THP1 THP2 HPE9 |
|---|---|
| Target | Gli2 |
| Molecular Weight(MW) | 168 KDa (note) |
Application
IHC-P analysis of human heart tissue using Gli2 antibody at 4.0-8.0μg/ml.
Application
IHC-P analysis of human intestine tissue using Gli2 antibody at 4.0-8.0μg/ml.
Application
WB analysis of HepG2 cells using Gli2 antibody at 1.0μg/ml.
Application
IHC-Fr analysis of mouse gut tissue using Gli2 antibody at 1:50. Green : GL2 Red : Neural crest cell. Fixed tissue was treated with 0.1% Triton X-100 for 4-6 hours at room temperature or overnight at 4°C, washed in PBS, incubated with primary antibodies for 4-6 hours at room temperature or overnight at 4°C, washed in PBS, and then stained with secondary antibody (Goat Anti-Rabbit Cy3) for 4-6 hours at room temperature or overnight at 4°C.
Application
WB analysis of Jurkat cells using Gli2 antibody at 2.5μg/ml. Lane A : primary antibody Lane B : primary antibody + blocking peptide| Positive Control | HepG2 , Jurkat |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. For short-term storage, store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
