Application
Antibody used in WB on Jurkat 2.5 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).Application
Antibody used in WB on Human HepG2 at 1 ug/ml.Application
Antibody used in IHC on Human Heart at 4.0-8.0 ug/ml.Application
Antibody used in IHC on Mouse Gut at 1:50.Application
Antibody used in IHC on Human Intestine at 4.0-8.0 ug/ml.| Product Name | GLI2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GLI2, HPE9, THP1, THP2 |
| Product description | GLI2 encodes a protein which belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B.1) Ikram, M.S., et al., (2004) J. Invest. Dermatol. 122 (6), 1503-1509. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GLI2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| GLI2 antibody can be used for detection of GLI2 by ELISA at 1:312500. GLI2 antibody can be used for detection of GLI2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | GLI2 |
|---|---|
| Alternative Names | GLI2 HPE9 THP1 THP2 |
| Molecular Weight(MW) | 133 kDa |
Application
Antibody used in WB on Jurkat 2.5 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).
Application
Antibody used in WB on Human HepG2 at 1 ug/ml.
Application
Antibody used in IHC on Human Heart at 4.0-8.0 ug/ml.
Application
Antibody used in IHC on Mouse Gut at 1:50.
Application
Antibody used in IHC on Human Intestine at 4.0-8.0 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | GLI2 antibody can be used for detection of GLI2 by ELISA at 1:312500. GLI2 antibody can be used for detection of GLI2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store GLI2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
