Application
WB analysis of lysates from HeLa cells transfected with siRNA directed against the H2B E3 ubiquitin ligase (hBRE1) or LacZ using Histone H2B (ubiquitinated) antibody [NRO3]. GAPDH antibody was used as loading control.| Product Name | Histone H2B (ubiquitinated) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [NRO3] to Histone H2B (ubiquitinated)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2B family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Jul 2008] |
| Immunogen | Ubiquitinated Histone H2B. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | NRO3 |
| Host Species | Mouse |
| Tested Applications | WBChIP |
| WB: 2 - 3 ug/ml: |
|
| Species Reactivity | HumanMouseDrosophilaArabidopsis |
| Concentration | 1mg/ml |
| Gene Synonyms | H2BQ H2BFQ H2BGL105 GL105 HIST2H2BE histone cluster 2 H2be H2B H2B.1 |
|---|---|
| Target | Histone H2B (ubiquitinated) |
Application
WB analysis of lysates from HeLa cells transfected with siRNA directed against the H2B E3 ubiquitin ligase (hBRE1) or LacZ using Histone H2B (ubiquitinated) antibody [NRO3]. GAPDH antibody was used as loading control.| Application Notes | WB: 2 - 3 ug/ml: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Lyophilized antibody can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage. To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20ºC) or for long-term storage (-80ºC). |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
