Application
Histone H4K8ac (acetyl Lys8) antibody [GT478] detects Histone H4K8ac (acetyl Lys8) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4K8ac (acetyl Lys8) protein stained by Histone H4K8ac (acetyl Lys8) antibody [GT478] diluted at 1:400. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:300. Scale bar = 10 μm.Application
Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4K8ac (acetyl Lys8) antibody diluted at 1:1000.Application
Histone H4K8ac (acetyl Lys8) antibody [GT478] detects Histone H4K8ac (acetyl Lys8) protein at nucleus in human ovary by immunohistochemical analysis. Sample: Paraffin-embedded human ovary. Histone H4K8ac (acetyl Lys8) antibody [GT478] diluted at 1:200.Application
Histone H4K8ac (acetyl Lys8) antibody [GT478] detects Histone H4K8ac (acetyl Lys8) protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Histone H4K8ac (acetyl Lys8) antibody [GT478] diluted at 1:200.Application
Histone H4K8ac (acetyl Lys8) antibody [GT478] detects Histone H4K8ac (acetyl Lys8) protein at cytoplasm and nucleus in rat kidney by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. Histone H4K8ac (acetyl Lys8) antibody [GT478] diluted at 1:200.| Product Name | Histone H4K8ac (acetyl Lys8) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT478] to Histone H4 (acetyl Lys8) (histone cluster 1, H4a)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H4. The exact sequence is proprietary. |
| Modification | acetyl Lys8 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG3 |
| Clone Number | GT478 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevisXenopus laevis (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | H4FA antibody HIST1H4A antibody Histone H4 antibody Histone H4K8ac (acetyl Lys8) antibody [GT478] antibody histone cluster 1 H4a antibody |
|---|---|
| Target | Histone H4 |
| Molecular Weight(MW) | 11 kDa (note) |
| Cellular Localization | Nucleus |
Application
Histone H4K8ac (acetyl Lys8) antibody [GT478] detects Histone H4K8ac (acetyl Lys8) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4K8ac (acetyl Lys8) protein stained by Histone H4K8ac (acetyl Lys8) antibody [GT478] diluted at 1:400. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:300. Scale bar = 10 μm.
Application
Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4K8ac (acetyl Lys8) antibody diluted at 1:1000.
Application
Histone H4K8ac (acetyl Lys8) antibody [GT478] detects Histone H4K8ac (acetyl Lys8) protein at nucleus in human ovary by immunohistochemical analysis. Sample: Paraffin-embedded human ovary. Histone H4K8ac (acetyl Lys8) antibody [GT478] diluted at 1:200.
Application
Histone H4K8ac (acetyl Lys8) antibody [GT478] detects Histone H4K8ac (acetyl Lys8) protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Histone H4K8ac (acetyl Lys8) antibody [GT478] diluted at 1:200.
Application
Histone H4K8ac (acetyl Lys8) antibody [GT478] detects Histone H4K8ac (acetyl Lys8) protein at cytoplasm and nucleus in rat kidney by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. Histone H4K8ac (acetyl Lys8) antibody [GT478] diluted at 1:200.| Positive Control | HeLa , HeLa (0.4μM Trichostatin A for 18 hr) |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
