Application
Immunohistochemistry staining with anti-human HLA-G (MEM-G/1) . Fig. 1B - first-trimester placenta (paraffin-embedded sections)Application
The antibody stains infiltrating macrophages in pulmonary diseases. In the top left corner see detail of macropage.| Product Name | HLA-G antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [MEM - G / 1] to HLA GHLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq] |
| Immunogen | Recombinant full length denatured heavy chain (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | MEM-G/1 |
| Host Species | Mouse |
| Tested Applications | IHCIHC-PWB |
| IHC-P: Use at an assay dependant dilution. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. WB: Use at an assay dependant dilution. Does not react with native HLA-G on cell surface. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HLA G HLAG MHC-G MHC G MHCG |
|---|---|
| Target | HLA-G |
| Tissue Specificity | The antibody reacts with denaturated HLA-G heavy chain. |
| Cellular Localization | Cell Membrane |
Application
Immunohistochemistry staining with anti-human HLA-G (MEM-G/1) . Fig. 1B - first-trimester placenta (paraffin-embedded sections)
Application
The antibody stains infiltrating macrophages in pulmonary diseases. In the top left corner see detail of macropage.| Application Notes | IHC-P: Use at an assay dependant dilution. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. WB: Use at an assay dependant dilution. Does not react with native HLA-G on cell surface. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
