Application
Western blot analysis of HLA-G by the antibody on HLA-G1 transfectants (LCL-HLA-G1).| Product Name | HLA-G antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [MEM - G / 4] to HLA GHLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq] |
| Immunogen | Recombinant human HLA-G denaturated heavy chain. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | MEM-G/4 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB: Use at an assay dependent dilution. Predicted molecular weight: 38 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HLA G HLAG MHC-G MHC G MHCG |
|---|---|
| Target | HLA-G |
| Tissue Specificity | The antibody reacted with denaturated HLA - heavy chain. The recognized epitope is present only on HLA G1, G2 and G5 molecules. |
| Cellular Localization | Cell Membrane |
Application
Western blot analysis of HLA-G by the antibody on HLA-G1 transfectants (LCL-HLA-G1).| Application Notes | WB: Use at an assay dependent dilution. Predicted molecular weight: 38 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
