Application
Surface staining of JEG-3 human choriocarcinoma cell line with anti-human HLA-G: FITC . Total viable cells were used for analysis.| Product Name | HLA-G antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [MEM - G / 11] to HLA GHLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq] |
| Immunogen | Recombinant full length protein (Human) refolded with beta 2 microglobulin and peptide. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | MEM-G/11 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSIP |
| ELISA: Use at an assay dependant dilution. FACS: Use at an assay dependant dilution. IP: Use at an assay dependant dilution. Does not cross-block MEM-G/9 (GTX27758 and GTX27904). Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | HLA G HLAG MHC-G MHC G MHCG |
|---|---|
| Target | HLA-G |
| Tissue Specificity | HLA G antigen. |
| Cellular Localization | Cell Membrane |
Application
Surface staining of JEG-3 human choriocarcinoma cell line with anti-human HLA-G: FITC . Total viable cells were used for analysis.| Application Notes | ELISA: Use at an assay dependant dilution. FACS: Use at an assay dependant dilution. IP: Use at an assay dependant dilution. Does not cross-block MEM-G/9 (GTX27758 and GTX27904). Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8ºC. Do not freeze. Do not use after expiration date stamped on vial label. |
| Storage Buffer | Preservative: Sodium Azide; Constituents: 15mM PBS. pH 7.4 |
