Application
Immunofluorescent analysis of Hydrogen/Potassium ATPase beta in A2058 cells. Hydrogen/Potassium ATPase beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with Hydrogen/Potassium ATPase beta antibody [2G11] at a dilution of 1:100 over night at 4?C, washed with PBS and incubated with a proper secondary antibody. Images were taken at 60X magnification.| Product Name | Hydrogen/Potassium ATPase beta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [2G11] to Hydrogen/Potassium ATPase betaThe protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes the beta subunit of the gastric H+, K+-ATPase. [provided by RefSeq, Jul 2008] |
| Immunogen | Purified 34 kDa core peptide from deglycosylated hog gastric microsomes. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2G11 |
| Host Species | Mouse |
| Tested Applications | BlockingFACSICC/IFIHCIHC-FIHC-PIPWBChIP |
| ICC/IF: 1:3 - 1:3000: |
|
| Species Reactivity | HumanMouseDogPigRatGuinea PigRabbitBovineMink(Mustela) |
| Concentration | 1mg/ml |
| Gene Synonyms | ATP6B ATPase H+/K+ exchanging beta polypeptide ATP4B |
|---|---|
| Target | Hydrogen+Potassium ATPase beta |
Application
Immunofluorescent analysis of Hydrogen/Potassium ATPase beta in A2058 cells. Hydrogen/Potassium ATPase beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with Hydrogen/Potassium ATPase beta antibody [2G11] at a dilution of 1:100 over night at 4?C, washed with PBS and incubated with a proper secondary antibody. Images were taken at 60X magnification.| Application Notes | ICC/IF: 1:3 - 1:3000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20° C, Avoid Freeze/Thaw Cycles |
| Storage Buffer | Ascites containing 0.05 % sodium azide |
