WB analysis of (1) protein marker, (2) recombinant protein IL6, (3) 293T lysate and (4) SP2/0 lysate using IL6 antibody [1B1-B10-D2] at a concentration of 1μg/ml.
Mouse monoclonal antibody [1B1-B10-D2] to IL6This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq, Jun 2011]
Immunogen
Mouse Anti-Human Interleukin-6 Antibody
Key Feature
Clonality
Monoclonal
Isotype
IgG
Clone Number
1B1-B10-D2
Host Species
Mouse
Tested Applications
ELISAWB
WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity
Human
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
The mouse anti-human IL-6 antibody specifically detects targets derived from human recombinant protein at various concentrations. Cross reactivity with mouse and rat are expected from sequence similarity.
Application
Application
WB analysis of (1) protein marker, (2) recombinant protein IL6, (3) 293T lysate and (4) SP2/0 lysate using IL6 antibody [1B1-B10-D2] at a concentration of 1μg/ml.
Application Notes
WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form
Liquid
Storage Instructions
Store at 4°C if intended for use within one month, otherwise, store at -20 °C to -80 °C. The lyophilized antibody is stable for at least 18 months after the date of receipt when stored at -20 °C to -80°C. After reconstitution, it can be aliquoted and stor