Application
Sandwich ELISA analysis of human IL6 at 400, 160, 64, 25.6, 10.24, and 0 pg/ml (50 ul per well) using IL-6 antibody [7IL6] as capture antibody (4 ug/ml) and IL-6 antibody [5IL6] (Biotin) as detection antibody (0.035 ug/ml). The plate was washed and incubated with Streptavidin-HRP. Detection was performed using TMB substrate. The plate was stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550 nm.| Product Name | IL6 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [5IL6] to IL6This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq, Jun 2011] |
| Immunogen | Recombinant human IL-6. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 5IL6 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFWB |
| ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Synonyms | HSF BSF2 interleukin 6 (interferon beta 2) IL6 IFNB2 HGF |
|---|---|
| Target | IL6 |
Application
Sandwich ELISA analysis of human IL6 at 400, 160, 64, 25.6, 10.24, and 0 pg/ml (50 ul per well) using IL-6 antibody [7IL6] as capture antibody (4 ug/ml) and IL-6 antibody [5IL6] (Biotin) as detection antibody (0.035 ug/ml). The plate was washed and incubated with Streptavidin-HRP. Detection was performed using TMB substrate. The plate was stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550 nm.| Application Notes | ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
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| Form | Liquid |
|---|---|
| Storage Instructions | -20°C |
| Storage Buffer | PBS |
