Application
Melanoma cells transfected with human Integrin av and wild-type (WT) or mutant (Y773F) human Integrin beta3 were analyzed. Integrin beta3 was immunoprecipitated then incubated with Integrin beta3 [pY773] polyclonal antibody along with lysates corresponding to non-phosphorylated Integrin beta3 (1), phosphorylated WT Integrin beta3 (2), or phosphorylated Y773F mutant Integrin beta3 (3). Elimination of signal in site-directed mutant demonstrates antibody specificity.| Product Name | Integrin beta 3 (phospho Tyr773) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Integrin beta 3 ( phospho Y773 )Integrin beta 3, also known as CD61, is a 130 kDa transmembrane glycoprotein that binds noncovalently in complexes with integrin alpha subunits (alpha IIb, alpha v) to form the functional receptor that binds to specific extracellular matrix proteins (e.g., fibronectin, vitronectin, etc). Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. The clustering of integrin receptors on the cell surface and their binding to the extracellular matrix leads to the formation of focal contacts and the activation of various signal transduction pathways. Phosphorylation of tyrosine 773 on Integrin beta 3 activates the receptor and promotes cell adhesion. Tyr773 is commonly referred to as Tyr747, the corresponding site in the chicken Integrin beta 3 protein. |
| Immunogen | The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Integrin beta 3 receptor that contains tyrosine 773 (based on Swiss Protein database, accession number P05106). The sequence is conserved in human, |
| Modification | p-Tyr773 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a concentration of 0.1 - 1.0 μg/ml. Predicted molecular weight: 130 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | HumanMouseRatXenopus |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CD61 GP3A GPIIIa Integrin beta 3 (phospho Y773) Integrin beta 3 (phospho Tyr773) Integrin beta 3 (pY773) Integrin beta 3 (pTyr773) Integrin beta 3 phospho Y773 Integrin beta 3 phospho Tyr773 Phospho Integrin beta 3 (Y773) Phospho Integrin beta 3 |
|---|---|
| Target | Integrin beta 3 |
| Cellular Localization | Cell Membrane |
Application
Melanoma cells transfected with human Integrin av and wild-type (WT) or mutant (Y773F) human Integrin beta3 were analyzed. Integrin beta3 was immunoprecipitated then incubated with Integrin beta3 [pY773] polyclonal antibody along with lysates corresponding to non-phosphorylated Integrin beta3 (1), phosphorylated WT Integrin beta3 (2), or phosphorylated Y773F mutant Integrin beta3 (3). Elimination of signal in site-directed mutant demonstrates antibody specificity.| Positive Control | K562 cells transfected with human Integrin alpha and wild-type (WT) or mutant (Y773F) human Integrin beta 3, and analysed by immunoprecipitation with 1A2 (human Integrin beta 3) mAb |
|---|---|
| Application Notes | WB: Use at a concentration of 0.1 - 1.0 μg/ml. Predicted molecular weight: 130 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: PBS, 1mg/ml BSA (IgG, protease free). pH 7.3 |
