Application
K562 cells transfected with human Integrin αv and wild-type (WT) or mutant (Y785F) human Integrin β3 were analyzed by immunoprecipitating the Integrin.| Product Name | Integrin beta 3 (phospho Tyr785) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Integrin beta 3 ( phospho Y785 )Integrin beta 3, also known as CD61, is a 130 kDa transmembrane glycoprotein that binds noncovalently in complexes with integrin alpha subunits (alpha IIb, alpha v) to form the functional receptor that binds to specific extracellular matrix proteins (e.g., fibronectin, vitronectin, etc). Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. The clustering of integrin receptors on the cell surface and their binding to the extracellular matrix leads to the formation of focal contacts and the activation of various signal transduction pathways. Phosphorylation of tyrosine 785 on Integrin beta 3 is essential for Shc and Grb2 binding, and promotes cell migration. Tyr 785 is commonly referred to as Tyr 759, the corresponding site in the chicken Integrin beta 3 protein. |
| Immunogen | The antiserum was produced against a chemically synthesized phosphopeptide derived from the c-terminus of the Integrin beta 3 receptor (based on Swiss Protein database, accession number P05106). The sequence is conserved in human, mouse and rat. |
| Modification | p-Tyr785 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a concentration of 0.1 - 1.0 μg/ml. Predicted molecular weight: 130 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CD61 GP3A GPIIIa Integrin beta 3 (phospho Y785) Integrin beta 3 (phospho Tyr785) Integrin beta 3 (pY785) Integrin beta 3 (pTyr785) Integrin beta 3 phospho Y785 Integrin beta 3 phospho Tyr785 Phospho Integrin beta 3 (Y785) Phospho Integrin beta 3 |
|---|---|
| Target | Integrin beta 3 |
| Cellular Localization | Cell Membrane |
Application
K562 cells transfected with human Integrin αv and wild-type (WT) or mutant (Y785F) human Integrin β3 were analyzed by immunoprecipitating the Integrin.| Positive Control | K562 cells transfected with human Integrin alpha v and wild-type (WT) or mutant (Y785F) human Integrin beta 3, and analysed by immunoprecipitation with 1A2 (human Integrin beta 3) mAb |
|---|---|
| Application Notes | WB: Use at a concentration of 0.1 - 1.0 μg/ml. Predicted molecular weight: 130 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: PBS, 1mg/ml BSA (IgG, protease free). pH 7.3 |
