Application
Immunofluorescence staining of lamin C (green) in mouse EL-6 fibroblasts by monoclonal antibody EM-11 . DNA stained by DAPI (blue).Application
Immunofluorescence staining of lamin C (red) in HeLa cells by monoclonal antibody EM-11 . Actin labeled by phalloidin-Alexa Fluor® 488 (green), DNA stained by DAPI (blue).| Product Name | Lamin C antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [EM-11] to Lamin CLamin C is intermediate filament protein localized to the inner nuclear membrane. It is expressed predominantly in terminally differentiated cells and defines the shape and stability of nuclei in mammalian cells. Besides their structural roles, lamin proteins also regulate fundamental aspects of nuclear function and they cross-talk with cell signaling cascades and cell metabolism. Mutations of LMNA gene, encoding lamin A and C proteins, are often associated with pathogenesis of respective cell types, such as of heart myocytes.Entrez Gene (human): 1q21.2-q21.3 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | EM-11 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIPWB |
| ICC/IF: Note:Paraformaldehyde fixation possible: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Synonyms | CMD1A CMT2B1 EMD2 FPL FPLD HGPS LDP1 LFP LGMD1B LMN1 LMNC PRO1 |
|---|---|
| Target | Lamin C |
| Tissue Specificity | The mouse monoclonal antibody EM-11 recognizes lamin C, intermediate filament protein of nuclear lamina. |
| Cellular Localization | Farnesylation of prelamin-A/C facilitates nuclear envelope targeting and subsequent cleaveage by ZMPSTE24/FACE1 to remove the farnesyl group produces mature lamin-A/C, which can then be inserted into the nuclear lamina. EMD is required for proper localiza |
Application
Immunofluorescence staining of lamin C (green) in mouse EL-6 fibroblasts by monoclonal antibody EM-11 . DNA stained by DAPI (blue).
Application
Immunofluorescence staining of lamin C (red) in HeLa cells by monoclonal antibody EM-11 . Actin labeled by phalloidin-Alexa Fluor® 488 (green), DNA stained by DAPI (blue).| Application Notes | ICC/IF: Note:Paraformaldehyde fixation possible: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Storage Buffer | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
