| Product Name | Lamin C antibody, C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Lamin C (C-term)An important part of the cell nucleus is formed by nuclear lamina. Nuclear lamins form a network of filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. The nuclear lamins comprise a unique subclass of the intermediate filament protein family. They share a molecular domain organisation with the other intermediate filament proteins in that they are fibrous molecules that have an aminoterminal globular head, a central rod of a-helices and a carboxyterminal globular domain. Many biochemical and molecular features of lamins have been studied, but their functions remain still largely undetermined. One of the functions ascribed to the lamina is the maintenance of the structural integrity of the nucleus. Besides interactions with the nuclear membrane and other intermediate filaments, lamins interact with the nuclear chromatin. Eukaryotic chromatin is organised into loops, which are attached to the nuclear matrix. This organisation is thought to contribute to compaction of the chromatin and regulation of gene expression. Lamins, as part of the nuclear matrix, may be involved in these processes since chromatin binding sites have been detected in both A- and B-type lamins. |
| Immunogen | A peptide made from the last 8 amino acids of lamin C, including an N-terminal lysine as a linker (KHHVSGSRR), coupled to KLH |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| Optimal dilutions should be determined experimentally by the researcher.: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 70 kDa lamin Cardiomyopathy dilated 1A CDCD1 CDDC CMD1A CMT2B1 EMD2 FPL FPLD HGPS |
|---|---|
| Target | Lamin C |
| Tissue Specificity | This antibody reacts strongly in immunoblotting with fragments corresponding to peptide 319-572aa of Lamin C. When tested against nuclear protein extracts from HeLa cells it reacts with a single band migrating at 65 kDa. Stains lamin C from human, mouse and rat material |
| Cellular Localization | Farnesylation of prelamin-A/C facilitates nuclear envelope targeting and subsequent cleaveage by ZMPSTE24/FACE1 to remove the farnesyl group produces mature lamin-A/C, which can then be inserted into the nuclear lamina. EMD is required for proper localiza |
| Application Notes | Optimal dilutions should be determined experimentally by the researcher.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8°C. For extended storage, aliquot and store at -20°C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | Antiserum containing PBS + 0.1% sodium azide |
