| Product Name | Lamin A + C antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [JoL5] to Lamin A + CNuclear Lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type Lamins and B-type Lamins. The A-type Lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. Lamin A, Lamin C and Lamin Adel 10, while the B-type Lamins include two proteins arising from two distinct genes, i.e. Lamin B1 and Lamin B2. Recent evidence has revealed that mutations in A-type Lamins give rise to a range of rare but dominant genetic disorders, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease and Dunnigan-type familial partial lipodystrophy. In addition, the expression of A-type Lamins coincides with cell differentiation and as A-type Lamins specifically interact with chromatin, a role in the regulation of differential gene expression has been suggested for A-type Lamins. |
| Immunogen | Recombinant bacterially expressed human lamin A |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | JoL5 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| For WB: Use at 1/50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CDCD1 CDDC CMD1A CMT2B1 EMD2 FPL FPLD HGPS IDC LDP1 LFP LGMD1B LMN1 LMNC LMNL1 PRO1 |
|---|---|
| Target | Lamin A + C |
| Tissue Specificity | Detects an un-phosphorylated form of lamin A/C and the epitope is mapped to the N-terminal head domain of both proteins. Works on human & mouse |
| Cellular Localization | Farnesylation of prelamin-A/C facilitates nuclear envelope targeting and subsequent cleaveage by ZMPSTE24/FACE1 to remove the farnesyl group produces mature lamin-A/C, which can then be inserted into the nuclear lamina. EMD is required for proper localiza |
| Application Notes | For WB: Use at 1/50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8°C. For extended storage, aliquot and store at -20°C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | Tissue culture supernatant containing 0.1% sodium azide |
