Application
Formalin fixed paraffin embedded human tonsil stained with MCM7, using ABC and DAB chromagen.Application
Formalin-fixed, paraffin-embedded human tonsil stained with using peroxidase-conjugate and DAB chromogen. Note nuclear staining of proliferating cells.| Product Name | MCM7 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [47DC141] to MCM7hCDC47 is a human member of the MCM family, which allows DNA to replicate once per cell cycle. In quiescent cells, human MCM7 (hMCM7) mRNA is almost undetectable. Stimulation of cells to enter the cell cycle results in induction of hMCM7 expression. The hCDC47 protein expression and localization is found in nuclei of the proliferative components of normal lymph nodes, bone marrow, epidermis and mucosa. Malignant tumors from several organs contained more nuclearhCDC47 positive cells than their normal counterparts. These results indicate that hCDC47 may play a role in normal and neoplastic cell growth in vivo. |
| Immunogen | Full length recombinant human MCM7 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 47DC141 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PIPWB |
| ICC/IF:1:20~1:100 IHC-p:1:10~1:50 WB:1:100~1:200 IP:1:20~1:100 |
|
| Species Reactivity | HumanMouseDogRatXenopus |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CDC47 MCM2 P1.1-MCM3 P1CDC47 P85MCM PNAS146 P1.1 MCM3 P1.1MCM3 |
|---|---|
| Target | MCM7 |
| Cellular Localization | Nuclear . |
Application
Formalin fixed paraffin embedded human tonsil stained with MCM7, using ABC and DAB chromagen.
Application
Formalin-fixed, paraffin-embedded human tonsil stained with using peroxidase-conjugate and DAB chromogen. Note nuclear staining of proliferating cells.| Positive Control | Breast carcinoma |
|---|---|
| Application Notes | ICC/IF:1:20~1:100 IHC-p:1:10~1:50 WB:1:100~1:200 IP:1:20~1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | PBS, pH7.4 containg 0.2% BSA and 0.09% sodium azide |
