Application
Immunofluorescence of MDA5 in Human Lymph Node cells with MDA5 antibody at 20 ug/mL.Application
Western blot analysis of MDA5 in Daudi cell lysate with MDA5 antibody at (A) 1, (B) 2 and (C) 4 ug/mL.Application
Immunohistochemistry of MDA5 in human lymph node tissue with MDA5 antibody at 5 ug/mL.| Product Name | MDA5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MDA5DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein that is upregulated in response to treatment with beta-interferon and a protein kinase C-activating compound, mezerein. Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation. Genetic variation in this gene is associated with diabetes mellitus insulin-dependent type 19. [provided by RefSeq, Jul 2012] |
| Immunogen | MDA5 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human MDA5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PWB |
| IF: 20 ug/mL: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | RLR-2 interferon induced with helicase C domain 1 MDA-5 Hlcd IDDM19 IFIH1 |
|---|---|
| Target | MDA5 |
Application
Immunofluorescence of MDA5 in Human Lymph Node cells with MDA5 antibody at 20 ug/mL.
Application
Western blot analysis of MDA5 in Daudi cell lysate with MDA5 antibody at (A) 1, (B) 2 and (C) 4 ug/mL.
Application
Immunohistochemistry of MDA5 in human lymph node tissue with MDA5 antibody at 5 ug/mL.| Application Notes | IF: 20 ug/mL: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Be stored at 4oC for three months and -20oC, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PBS containing 0.02% sodium azide. |
