Application
Western blot analysis using anti-MDM4 mAb against Hela (1), A549 (2) and A431 (3) cell lysate.Application
Confocal immunofluorescence analysis of Hela (left) and L-02 (right) cells using anti-MDM4 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.Application
Immunohistochemical analysis of paraffin-embedded human cerebra (left) and lung carcinoma (right) tissues, showing nuclear localization with DAB staining using anti-MDM4 mAb.| Product Name | MDMX antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [2D10F4] to MDM4This gene encodes a nuclear protein that contains a p53 binding domain at the N-terminus and a RING finger domain at the C-terminus, and shows structural similarity to p53-binding protein MDM2. Both proteins bind the p53 tumor suppressor protein and inhibit its activity, and have been shown to be overexpressed in a variety of human cancers. However, unlike MDM2 which degrades p53, this protein inhibits p53 by binding its transcriptional activation domain. This protein also interacts with MDM2 protein via the RING finger domain, and inhibits the latter's degradation. So this protein can reverse MDM2-targeted degradation of p53, while maintaining suppression of p53 transactivation and apoptotic functions. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Feb 2011] |
| Immunogen | The immunogen corresponding to human MDM4 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2D10F4 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIHCIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MRP1 MDMX HDMX |
|---|---|
| Target | MDM4 |
| Molecular Weight(MW) | 55 kDa (note) |
Application
Western blot analysis using anti-MDM4 mAb against Hela (1), A549 (2) and A431 (3) cell lysate.
Application
Confocal immunofluorescence analysis of Hela (left) and L-02 (right) cells using anti-MDM4 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.
Application
Immunohistochemical analysis of paraffin-embedded human cerebra (left) and lung carcinoma (right) tissues, showing nuclear localization with DAB staining using anti-MDM4 mAb.| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
