Application
Formalin fixed paraffin embedded human rhabdomyosarcoma stained with Myogenin antibody .Application
Formalin fixed paraffin embedded human rhabdomyosarcoma stained with Myogenin using ABC and AEC chromogen.| Product Name | Myogenin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [F5D] to MyogeninMyogenin is a member of a family of myogenic regulatory genes, which includes MyoD, myf5 and MRF4. These genes encode a set of transcription factors, which are essential for muscle development. Transfection of myogenin into multipotential mesodermal cells have been shown to convert the mesodermal cells to myoblasts. Expression of myogenin is restricted to cells of skeletal muscle origin. It is therefore a useful marker for tumors of the muscle lineage, being strongly expressed in alveolar rhabdomyosarcomas. |
| Immunogen | BALB / C mice were injected with recombinant protein containing rat myogenin amino acid 30 - 224. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | F5D |
| Host Species | Mouse |
| Tested Applications | GSAICC/IFIHC-FIHC-PIPWB |
| This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. We suggest an incubation period of 30 minutes at room temperature. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Optimal dilutions / concentrations should be determined by the end user.: |
|
| Species Reactivity | MouseRatCat |
| Concentration | 1mg/ml |
| Gene Synonyms | Myogenin P15173 4656 Myog 29148 MYF4 159980 |
|---|---|
| Target | Myogenin |
| Tissue Specificity | This antibody is specific to 34 kDa protein known as Myogenin. |
| Cellular Localization | Nuclear |
Application
Formalin fixed paraffin embedded human rhabdomyosarcoma stained with Myogenin antibody .
Application
Formalin fixed paraffin embedded human rhabdomyosarcoma stained with Myogenin using ABC and AEC chromogen.| Positive Control | Rhabdomyosarcoma |
|---|---|
| Application Notes | This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. We suggest an incubation period of 30 minutes at room temperature. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Optimal dilutions / concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containing 0.1% sodium azide |
