Application
ApplicationApplication
Immunohistochemcial staining of rhabdomyosrcoma using| Product Name | Myogenin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [SPM144] to MyogeninMyogenin is a member of a family of myogenic regulatory genes, which includes MyoD, myf5 and MRF4. These genes encode a set of transcription factors, which are essential for muscle development. Transfection of myogenin into multipotential mesodermal cells have been shown to convert the mesodermal cells to myoblasts. Expression of myogenin is restricted to cells of skeletal muscle origin. It is therefore a useful marker for tumors of the muscle lineage, being strongly expressed in alveolar rhabdomyosarcomas. |
| Immunogen | Recombinant protein encoding rat myogenin aa 30-224. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | SPM144 |
| Host Species | Mouse |
| Tested Applications | IHCIHC-PIPWB |
| IHC-P: Use at a dilution of 1/40 for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at RT for 20 minutes. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 25 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.2mg/ml |
| Purification | Protein G |
| Gene Synonyms | Myogenin P15173 4656 Myog 29148 MYF4 159980 |
|---|---|
| Target | Myogenin |
| Cellular Localization | Nuclear |
| Light Chain Type | kappa |
Application
Application
Application
Immunohistochemcial staining of rhabdomyosrcoma using| Positive Control | Rh30 cells. Rhabdomyosarcoma or Wilm's tumor. |
|---|---|
| Application Notes | IHC-P: Use at a dilution of 1/40 for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at RT for 20 minutes. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 25 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4 containing 1% BSA and 0.09% sodium azide |
