Application
Immunohistochemistry: Neurofibromin 1 Antibody - NF1 protein labeling displays positive signal in the maturing and the hypertrophic cartilages but only faint labeling in the proliferative cartilage.Application
Western Blot: Neurofibromin 1 Antibody - Western Blot of (A, B, C above).| Product Name | Neurofibromin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal [PcNFn27] to NeurofibrominNeurofibromin is a product of the tumor suppressor gene, Neurofibromatosis type I. Neurofibromin is known to have GTPase activity that modulates the ras pathway. The absence of or alteration of the neurofibromin protein may lead to Neurofibromatosis disease. This protein has not been purified, therefore, most of the information regarding this protein has been deduced from homology analysis of its gene sequence. |
| Immunogen | A highly conserved N-terminal peptide [QQNTHTKVSTEHNKE(C)], a.a 27-41 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Clone Number | PcNFn27 |
| Host Species | Rabbit |
| Tested Applications | IHCIHC-FIHC-PWB |
| For WB: Use at a dilution of 1:500 - 1:2,000. Can be used for Western blotting (band-set at 220-240 KD and a higher MW band at 450 KD (uncharacterized). For IHC: Use at a dilution of 1:200 - 1:500. This antibody does not require antigen retrieval methods. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseRatFishPuffer Fish(Fugu) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 162200 VRNF 4763 DKFZp686J1293 NFNS P21359 Neurofibromin WSS NF1 |
|---|---|
| Target | Neurofibromin |
| Tissue Specificity | This antibody is specific for neurofibromin. |
| Cellular Localization | Cytoplasmic |
Application
Immunohistochemistry: Neurofibromin 1 Antibody - NF1 protein labeling displays positive signal in the maturing and the hypertrophic cartilages but only faint labeling in the proliferative cartilage.
Application
Western Blot: Neurofibromin 1 Antibody - Western Blot of (A, B, C above).| Application Notes | For WB: Use at a dilution of 1:500 - 1:2,000. Can be used for Western blotting (band-set at 220-240 KD and a higher MW band at 450 KD (uncharacterized). For IHC: Use at a dilution of 1:200 - 1:500. This antibody does not require antigen retrieval methods. Optimal dilutions/concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline containing 0.02% sodium azide |
