Mouse Monoclonal antibody [3H11] to NF-MNeurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
Immunogen
Recombinant fusion protein containing the extreme C-terminus of rat NF-M expressed in and purified from E.coli.
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3H11
Host Species
Mouse
Tested Applications
ICC/IFWB
Immunofluorescence: Max Dilution 1/100:
Species Reactivity
HumanMouseChickenRatBovine
Concentration
1mg/ml
Target Information
Gene Synonyms
NEFM neurofilament medium polypeptide NF-M NEF3 NFM
Target
NF-M
Tissue Specificity
This antibody recognizes the carboxy terminus of neurofilament M (NF-M).
Application
Application
WB analysis of rat cortex tissue lysate using NF-M antibody [3H11], C-term.
Application Notes
Immunofluorescence: Max Dilution 1/100:
Additional Information
Form
Liquid
Storage Instructions
Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.