Application
Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with NG2 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | NG2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CSPG4 (chondroitin sulfate proteoglycan 4)A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human CSPG4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (86%), Rat (83%), Bovine (85%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NG2 MEL CSPG HMW MAA MCSP MCSPG chondroitin sulfate proteoglycan 4 MSK16 |
|---|---|
| Target | CSPG4 |
| Molecular Weight(MW) | 251 kDa (note) |
| Cellular Localization | Apical cell membrane; Single-pass type I membrane protein; Extracellular side , Cell projection , lamellipodium membrane; Single-pass type I membrane protein; Extracellular side , Apical cell membrane , Cell projection , lamellipodium membrane |
Application
Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with NG2 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | A375 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
